diff --git a/src/ModelContextProtocol.AspNetCore/StreamableHttpHandler.cs b/src/ModelContextProtocol.AspNetCore/StreamableHttpHandler.cs
index f7db4630e..0d3cf3240 100644
--- a/src/ModelContextProtocol.AspNetCore/StreamableHttpHandler.cs
+++ b/src/ModelContextProtocol.AspNetCore/StreamableHttpHandler.cs
@@ -704,8 +704,8 @@ private static string[] GetConfiguredSupportedProtocolVersions(string? protocolV
     }
 
     /// <summary>
-    /// Validates that HTTP requests using per-request metadata declare the same protocol version in both
-    /// the <c>MCP-Protocol-Version</c> header and body <c>_meta</c> envelope.
+    /// Validates that HTTP requests declare matching protocol versions in the
+    /// <c>MCP-Protocol-Version</c> header and the corresponding body field.
     /// </summary>
     private static bool ValidateProtocolVersionEnvelope(
         HttpContext context,
@@ -719,6 +719,18 @@ private static bool ValidateProtocolVersionEnvelope(
         }
 
         var protocolVersionHeader = context.Request.Headers[McpProtocolVersionHeaderName].ToString();
+
+        if (message is JsonRpcRequest { Method: RequestMethods.Initialize, Params: JsonObject initializeParams } &&
+            initializeParams["protocolVersion"] is JsonValue initializeProtocolVersionValue &&
+            initializeProtocolVersionValue.TryGetValue(out string? initializeProtocolVersion) &&
+            !string.IsNullOrEmpty(protocolVersionHeader) &&
+            !string.Equals(protocolVersionHeader, initializeProtocolVersion, StringComparison.Ordinal))
+        {
+            errorDetail = CreateHeaderMismatchError(
+                $"Bad Request: The {McpProtocolVersionHeaderName} header value '{protocolVersionHeader}' does not match body params.protocolVersion value '{initializeProtocolVersion}'.");
+            return false;
+        }
+
         bool hasProtocolVersionMeta = TryGetProtocolVersionMeta(message, out var protocolVersionMeta);
 
         if (!McpProtocolVersions.RequiresPerRequestMetadata(protocolVersionHeader) &&
diff --git a/tests/ModelContextProtocol.AspNetCore.Tests/StreamableHttpServerConformanceTests.cs b/tests/ModelContextProtocol.AspNetCore.Tests/StreamableHttpServerConformanceTests.cs
index 2bdd9d7e6..3422405a6 100644
--- a/tests/ModelContextProtocol.AspNetCore.Tests/StreamableHttpServerConformanceTests.cs
+++ b/tests/ModelContextProtocol.AspNetCore.Tests/StreamableHttpServerConformanceTests.cs
@@ -204,6 +204,31 @@ public async Task PostRequest_Succeeds_WithValidProtocolVersionHeader()
         Assert.Equal(HttpStatusCode.OK, response.StatusCode);
     }
 
+    [Theory]
+    [InlineData(false, McpProtocolVersions.March2025ProtocolVersion, McpProtocolVersions.November2025ProtocolVersion)]
+    [InlineData(false, McpProtocolVersions.November2025ProtocolVersion, McpProtocolVersions.March2025ProtocolVersion)]
+    [InlineData(true, McpProtocolVersions.March2025ProtocolVersion, McpProtocolVersions.November2025ProtocolVersion)]
+    [InlineData(true, McpProtocolVersions.November2025ProtocolVersion, McpProtocolVersions.March2025ProtocolVersion)]
+    public async Task InitializeRequest_IsBadRequest_WhenProtocolVersionHeaderDoesNotMatchBody(
+        bool stateless,
+        string protocolVersionHeader,
+        string protocolVersionBody)
+    {
+        await StartAsync(stateless);
+
+        var body = $$$$"""
+            {"jsonrpc":"2.0","id":4242,"method":"initialize","params":{"protocolVersion":"{{{{protocolVersionBody}}}}","capabilities":{},"clientInfo":{"name":"IntegrationTestClient","version":"1.0.0"}}}
+            """;
+        using var request = new HttpRequestMessage(HttpMethod.Post, "") { Content = JsonContent(body) };
+        request.Headers.Add("MCP-Protocol-Version", protocolVersionHeader);
+        using var response = await HttpClient.SendAsync(request, TestContext.Current.CancellationToken);
+
+        Assert.Equal(HttpStatusCode.BadRequest, response.StatusCode);
+        var json = JsonNode.Parse(await response.Content.ReadAsStringAsync(TestContext.Current.CancellationToken));
+        Assert.Equal(4242, json!["id"]!.GetValue<long>());
+        Assert.Equal((int)McpErrorCode.HeaderMismatch, json["error"]!["code"]!.GetValue<int>());
+    }
+
     [Fact]
     public async Task GetRequest_IsBadRequest_WithInvalidProtocolVersionHeader()
     {